北京大学海外学者讲学计划 | Spatially resolved single-cell epigenomics and functional genomics

　　主讲人：

　　Tian Lu (陆天)

　　Harvard University

　　主持人：

　　邢栋 研究员

　　会议时间：

　　2022年11月30日（星期三）9:00-10:00

　　腾讯会议链接：

　　https://meeting.tencent.com/dm/V1ZzsOKg6iu6

　　ID：646-120-529

　　会议密码：676390

　　Abstract:

　　The recent development of spatial omics methods, enables spatial profiling of transcriptome and 3D structure of genomes at a single-cell level. Expanding the repertoire of spatial omics tools, a spatial epigenomics method, as well as an pooled CRISPR screening approach, potentially compatible with spatial omics phenotype, will accelerate our understanding of the spatial regulation of cell and tissue functions. Here , we will first introduce a method which spatially profiled epigenomes in single cells and provided high-throughput and high-resolution measurements of spatial distributions of active promoters and putative enhancers in embryonic and adult mouse brains. Our results further revealed putative enhancer hubs regulating the expression of developmentally important genes. We will also describe an imaging-based pooled CRISPR screening approach that combines high-content phenotype imaging with single guide RNA (sgRNA) identification in individual cells. We used this approach to screen RNA-binding proteins for their effects on RNA localization to nuclear compartments and revealed both positive and negative regulators for the nuclear speckle localization of a long noncoding RNA, MALAT1, suggesting a dynamic regulation of lncRNA localization in subcellular compartments.