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Wensheng Wei, PI


T: +86-10-62757227
E: wswei@pku.edu.cn

604, Jingling Life Science Building, Peking University, Beijing 100871, China

Profile

  Wensheng Wei was born in 1969 at Jiangsu province, China. He received his bachelor degree of Biochemistry from Peking University in 1991, and Ph.D. degree of Genetics from Michigan State University in 1999. He then went to Prof. Stanley Cohen's lab at Stanford University School of Medicine for post-doctoral training, and became a research associate in 2005. Dr. Wei returned to Peking University in 2007 and became a principle investigator at the School of Life Sciences. He is currently jointly appointed as Investigator by Biomedical Pioneering Innovation Center (BIOPIC), Beijing Advanced Innovation Center for Genomics (ICG), and Peking-Tsinghua Center for Life Sciences (CLS).

  Dr. Wei has published a number of papers in high-profile journals, including Cell, Nature, Nature Biotechnology, and PNAS, as either first or corresponding author. He has been granted four patents, and is the recipient of the follow-ing awards: China Patent Award, Tan Jiazhen Life Science Award, Scientific Chinese Man of the Year, Zheng Changxue Teaching Award, BAYER Investigator Award, Roche Chinese Young Investigator Award, Peking University Dongbao Fellowship, and the most popular teacher of School of Life Sciences award.

  Dr. Wei's group is mainly focused on the development of eukaryotic genome editing technology, especially their applications in dynamic imaging techniques and high-throughput functional genomics. The combination of for-ward and reverse genetic means is employed, often in a high-throughput fashion, for the identification of host genes or non-coding RNAs important for host response during microbial infections or tumorigenesis.

  

  Selected Publications

  1. Zhu S, Cao Z, Liu Z, He Y, Wang Y, Yuan P, Li W, Tian F, Bao Y and Wei W*. (2019) Guide RNAs with embedded barcodes boost CRISPR-pooled screens. Genome Biol. 20, 20.
  2. Liu Y, Cao Z, Wang Y, Guo Y, Xu P, Yuan P, Liu Z, He Y and Wei W*. (2018) Genome-wide screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat. Biotechnol.36, 1203-1210.
  3. Zhang H, Zhou Y, Wang Y, Zhao Y, Qiu Y, Zhang X, Yue D, Zhou Z, and Wei W*. (2018). A surrogate reporter system for multiplexable evaluation of CRISPR/Cas9 in targeted mutagenesis. Sci Rep 8, 1042.
  4. Zhang Y, Liu L, Guo S, Song J, Zhu C, Yue Z, Wei W* and Yi C*. (2017) Deciphering TAL effectors for 5-methylcytosine and 5-hydroxymethylcytosine recognition. Nat Commun 8(1): 901.
  5. He R, Peng J, Yuan P, Yang J, Wu X, Wang Y andWei W*. (2017). Glucosyltransferase Activity of Clostridium difficile Toxin B Triggers Autophagy-mediated Cell Growth Arrest. Sci Rep 7(1): 10532.
  6. Zhou Y, Wang P, Tian F, Gao G, Huang L*, Wei W* and Xie X.S* (2017) Painting A Specific Chromosome with CRISPR/Cas9 for Live-cell Imaging. Cell Res. 27, 298-301.
  7. Zhou Y, Zhang H and Wei W*. (2016) Simultaneous generation of multi-gene knockouts in human cells. FEBS Letters 590, 4343-4353.
  8. Zhu S, Li W, Liu J, Chen C-H, Liao Q, Xu P, Xu H, Xiao T, Cao Z, Peng J, Yuan P, Brown M, Liu X* & Wei W*. (2016) Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR library. Nat Biotechnol 34, 1279-1286.
  9. He R, Peng J, Yuan P, Xu F, Wei W*. (2015) Divergent roles of BECN1 in LC3 lipidation and autophagosomal function. Autophagy 11:5, 740-747.
  10. Ren Q, Li C, Yuan P, Cai C, Luo G, Zhang L and Wei W*. (2015) A Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/ Cas9 Library Screening of the Hepatitis C Virus. Sci. Rep. 5, 8865.
  11. Yuan P, Zhang H, Cai C, Zhu S, Zhou Y, Yang X, He R, Li C, Guo S, Li S, Huang T, Feng H and Wei W*. (2015) Chondroitin sulfate proteoglycan 4 functions as the cellular receptor for Clostridium difficile toxin B. Cell Res. 25:157-168.