Professor Yanyi Huang received his BS (Chemistry) and ScD (Inorganic Chemistry) degrees from Peking University in 1997 and 2002, respectively. He then conducted his postdoc research at Caltech (Applied Physics, 2002-2005), and at Stanford (Bioengineering, 2005- 2006). He started his independent career at Peking University in 2006. He is PI of BIOPIC and ICG, Professor of Analytical Chemistry, and PI of Peking-Tsinghua Center for Life Sciences. Professor Huang has published over 150 papers. He has received National Natural Science Award, 2nd Rank, in 2003, the National Excellent Doctoral Thesis Award in 2004, the NSFC Award for Excellent Young Scholars in 2012, and the NSFC Award for Distinguished Young Scientist in 2015. He became Fellow of Royal Society of Chemistry in 2014.

　　Huang group is working on technology development for integrative biology researches, especially the new methods for genomic sequencing and large scale microfluidics. We are interested in developing advanced technologies to facilitate genome sequencing and its applications. Huang lab has developed numerous key components, structures, functional modules, and fabrication methods to enhance the complexity and sophistication of experiments that can be performed on a microfluidic chip. The advantages of these micro-scale building blocks enable quantitatively more and qualitatively new measurements, including the single copy measurements, of complex biological systems.

　　These developments enable researchers to perform experiments which are impossible or impractical to execute with traditional bench top techniques, and to provide innovative solutions to compelling problems which will yield fundamental insights into important biological problems. Huang lab has also developed microfluidic circuits for more precise characterization of heterogeneous systems at the single-cell resolution using high-throughput sequencing technologies, allowing cells to be identified objectively without a priori knowledge. Recently, Huang group has developed a new strategy to perform super- high accuracy DNA sequencing with information redundancy. This technology, ECC sequencing, will further facilitate the researches that requires accuracy that cannot be offered by current methods.

　　Selected Publications

1. Ruoyan Li,# Lin Di,# Jie Li,# Wenyi Fan,# Yachen Liu, Wenjia Guo, Weiling Liu, Lu Liu, Qiong Li, Liping Chen, Yamei Chen, Chuanwang Miao, Hongjin Liu, Yuqian Wang, Yuling Ma, Deshu Xu, Dongxin Lin*, Yanyi Huang*, Jianbin Wang*, Fan Bai*, Chen Wu*. A body map of somatic mutagenesis in morphologically normal human tissues. Nature 2021, 597 (7876), 398-403.

2. Lin Di, Yusi Fu, Yue Sun, Jie Li, Lu Liu, Jiacheng Yao, Guanbo Wang, Yalei Wu, Kaiqin Lao, Raymond W Lee, Genhua Zheng, Jun Xu, Juntaek Oh, Dong Wang, X. Sunney Xie*, Yanyi Huang*, Jianbin Wang*. RNA Sequencing by Direct Tagmentation of RNA/DNA Hybrids. PNAS 2020, 117, 2886-2893.

3. Xiannian Zhang, Tianqi Li, Feng Liu, Yaqi Chen, Jiacheng Yao, Zeyao Li, Yanyi Huang*, Jianbin Wang*. Comparative analysis of droplet-based ultra-high-throughput single-cell RNA-seq systems. Molecular Cell 2019, 73, 130-142.

4. Zitian Chen, Wenxiong Zhou, Shuo Qiao, Li Kang, Haifeng Duan, X. Sunney Xie, Yanyi Huang*. Highly accurate fluorogenic DNA sequencing with information theory-based error correction. Nature Biotechnology 2017, 35, 1170-1178.

5. Zitian Chen, Peiyu Liao, Fangli Zhang, Mengcheng Jiang, Yusen Zhu, Yanyi Huang*. Centrifugal micro-channel array droplet generator for highly parallel digital PCR. Lab Chip 2017, 17, 235-240

6. Yusi Fu, Chunmei Li, Sijia Lu, Wenxiong Zhou, Fuchou Tang, X. Sunney Xie*, Yanyi Huang*. Uniform and accurate single cell sequencing based on emulsion whole-genome amplification. PNAS 2015, 112, 11923-11928.

7. Jie Shen, Dongqing Jiang, Yusi Fu, Xinglong Wu, HongshanGuo, Binxiao Feng, Yuhong Pan g, Aaron M. Streets, Fuchou Tang*, Yanyi Huang*. H3K4me3 epigenomic landscape derived from ChIP-Seq of 1,000 mouse early embryonic cells. Cell Research 2015, 25, 143-147.