Yanyi Huang,

Associate Director

http://gene.pku.edu.cn
T: +86-10-62767408
E: yanyi@pku.edu.cn

Rm. 307, Integrated Science Research Center, Peking University, Beijing 100871, China

Profile

Professor Yanyi Huang received his BS (Chemistry) and ScD (Inorganic Chemistry) degrees from Peking University in 1997 and 2002, respectively. He then conducted his postdoc research at Caltech (Applied Physics, 2002-2005) with Amnon Yariv, and at Stanford (Bioengineering, 2005-2006) with Stephen Quake. He started his independent career at Peking University in 2006 as a Principal Investigator and then promoted to Associate Professor in 2009, and Professor in 2013. He is Principal Investigator in Beijing Advanced Innovation Center for Genomics (ICG), Professor of Materials Science and Engineering, Principal Investigator in Biodynamic Optical Imaging Center (BIOPIC), Principal Investigator in Peking-Tsinghua Center for Life Sciences, and an Adjunct Professor of Analytical Chemistry.  

Professor Huang has published over 120 papers. He has received National Natural Science Award, 2nd Rank, in 2003, the National Excellent Doctoral Thesis Award in 2004, the Fok Ying Tung Education Foundation Young Investigator Award in 2010, The NSFC Award for Excellent Young Scholars in 2012, and the NSFC Award for Distinguished Young Scientist in 2015. He became Fellow of Royal Society of Chemistry in 2014. He has been actively serving the community including sitting on the Advisory Board of Lab on a Chip, the Editorial Board of Biomicrofluidics, and the Features Panel of Analytical Chemistry. 

Huang group is working on technology development for integrative biology researches, especially the new methods for genomic sequencing and large scale mirofluidics. We are interested in developing advanced technologies to facilitate genome sequencing and its applications. Huang lab has developed numerous key components, structures, functional modules, and fabrication methods to enhance the complexity and sophistication of experiments that can be performed on a microfluidic chip. These miniaturized devices have been optimized to implement many specific bioanalysis. The advantages of these micro-scale building blocks enable quantitatively more and qualitatively new measurements, including the single copy measurements, of complex biological systems. These developments enable researchers to perform experiments which are impossible or impractical to execute with traditional bench top techniques, and to provide innovative solutions to compelling problems which will yield fundamental insights into important biological problems. Huang lab has also developed microfluidic circuits for more precise characterization of heterogeneous systems at the single-cell resolution using high-throughput sequencing technologies, allowing cells to be identified objectively without a priori knowledge. These microfluidic approaches facilitate single-cell manipulation, minimize contamination, eliminate operational errors, improve detection sensitivity, and increase experimental efficiency and throughput. 


Key Publications

1. Zitian  Chen, Peiyu Liao, Fangli Zhang, Mengcheng Jiang, Yusen Zhu, Yanyi Huang.*   Centrifugal micro-channel array droplet generator for highly parallel digital PCR. Lab Chip 2017, 17, 235-240.

2. Chen Cao,   Dong Zhou, Tao Chen, Aaron M. Streets,* Yanyi Huang.* Label-free Digital  Quantification of Lipid Droplets in Single Cells by Stimulated Raman Microscopy on a Microfluidic Platform. Anal.  Chem. 2016, 88, 4931-4939.

3. Yusi Fu, Chunmei Li, Sijia Lu, Wenxiong Zhou, Fuchou Tang, X. Sunney Xie,* Yanyi Huang.* Uniform and accurate single-cell sequencing based on emulsion  whole-genome amplification. PNAS 2015, 112, 11923-11928.

4. Jie Shen, Dongqing Jiang, Yusi Fu, Xinglong Wu, Hongshan Guo, Binxiao Feng, Yuhong Pang, Aaron M. Streets, Fuchou Tang,* Yanyi Huang.* H3K4me3 epigenomic landscape derived from ChIP-Seq of 1,000 mouse early embryonic cells. Cell Res. 2015, 25, 143-147.

5. Aaron M. Streets, Xiannian Zhang, Chen Cao, Yuhong Pang, Xinglong Wu, Liang Xiong,  Lu Yang, Yusi Fu, Liang Zhao,* Fuchou Tang,* Yanyi Huang.* Microfluidic single-cell whole transcriptome sequencing. PNAS 2014, 111, 7048-7053.